Journal: Scientific Reports

Article Title: Evaluation of candidate reference genes stability for gene expression analysis by reverse transcription qPCR in Clostridium perfringens

doi: 10.1038/s41598-022-23804-7

Figure Lengend Snippet: Candidate reference genes, functional groups, primer sequences, and expected amplicon sizes. Fragment sizes are based on alignment to C. perfringens ATCC 13124.

Article Snippet: Bacterial cultures were grown on perfringens agar (HiMedia, Kennett Square, PA) for routine maintenance and in BHI (Criterion, Hardy Diagnostics, Santa Maria, CA) broth during experimental assays.

Techniques: Functional Assay, Amplification, Sequencing

Journal: Scientific Reports

Article Title: Evaluation of candidate reference genes stability for gene expression analysis by reverse transcription qPCR in Clostridium perfringens

doi: 10.1038/s41598-022-23804-7

Figure Lengend Snippet: Relative expression of alpha toxin ( plc ) during growth of Clostridium perfringens over a 22-h period. The expression data were normalized using ftsZ , gyrA , recA , rrs , or tpiA as reference genes individually and with the geometric mean of ftsZ + gyrA + recA. 22-h samples were considered the control for calculations. P ≤ 0.05 shown by *

Article Snippet: Bacterial cultures were grown on perfringens agar (HiMedia, Kennett Square, PA) for routine maintenance and in BHI (Criterion, Hardy Diagnostics, Santa Maria, CA) broth during experimental assays.

Techniques: Expressing

Journal: Food Science and Biotechnology

Article Title: Evaluation of the microbial contamination of fresh produces and their cultivation environments from farms in Korea

doi: 10.1007/s10068-019-00570-3

Figure Lengend Snippet: Culture media a and identification methods for pathogenic bacteria isolated from fresh produce in this study

Article Snippet: Rappaport–Vassiliadis R10 broth (Difco) Tetrathionate broth (Difco) Xylose lysine desoxycholate agar (XLD, Difco) API 20E (BioMeriux) – – Staphylococcus aureus Tryptic soy broth (Difco) in 10% NaCl Baird-Parker agar (BPA, Difco) API Staphy (BioMeriux) – (SolGent co.,Ltd., Korea) Escherichia coli O157:H7 E.C. broth (Oxoid, Hampshire, UK) Sorbitol MacConkey agar (SMAC, Difco) – E. coli O157 Diagnostic Reagents test kit (Oxoid) – Clostridium perfringens Cooked meat (Difco) Perfringens agar base (TSC, Difco) – – – Open in a separate window a Culture media for pathogens quoted from the MFDS Food Code b The only three samples showing positive results on culture media methods for E. coli O157:H7 or L. monocytogenes were identified using Latex agglutination test method c Samples showing positive results with culture media methods were subsequently subjected to the 16s rRNA method d Not tested Culture media a and identification methods for pathogenic bacteria isolated from fresh produce in this study

Techniques: Isolation, Agglutination, Sequencing, Diagnostic Assay

Journal: Food Science and Biotechnology

Article Title: Evaluation of the microbial contamination of fresh produces and their cultivation environments from farms in Korea

doi: 10.1007/s10068-019-00570-3

Figure Lengend Snippet: Numbers of positive samples with pathogenic contamination of fresh produce

Article Snippet: Rappaport–Vassiliadis R10 broth (Difco) Tetrathionate broth (Difco) Xylose lysine desoxycholate agar (XLD, Difco) API 20E (BioMeriux) – – Staphylococcus aureus Tryptic soy broth (Difco) in 10% NaCl Baird-Parker agar (BPA, Difco) API Staphy (BioMeriux) – (SolGent co.,Ltd., Korea) Escherichia coli O157:H7 E.C. broth (Oxoid, Hampshire, UK) Sorbitol MacConkey agar (SMAC, Difco) – E. coli O157 Diagnostic Reagents test kit (Oxoid) – Clostridium perfringens Cooked meat (Difco) Perfringens agar base (TSC, Difco) – – – Open in a separate window a Culture media for pathogens quoted from the MFDS Food Code b The only three samples showing positive results on culture media methods for E. coli O157:H7 or L. monocytogenes were identified using Latex agglutination test method c Samples showing positive results with culture media methods were subsequently subjected to the 16s rRNA method d Not tested Culture media a and identification methods for pathogenic bacteria isolated from fresh produce in this study

Techniques: